Part:BBa_K4633006:Design

paraE - improved promoter for the araE gene from Bacillus subtilis 168

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The promoter was tested in B. subtilis 168 and in E. coli TOP10 using composite part BBa_K4633103 and the commercial plasmid pBE-S by Takara.

Source

The sequence was originally extracted from the work by Sá-Nogueira et al. [1] and then modified.

References

• [1] Sá-Nogueira, I., Nogueira, T. V., Soares, S. & De Lencastre, H. The Bacillus subtilis L-arabinose (ara) operon: Nucleotide sequence, genetic organization and expression. Microbiology 143, 957–969 (1997).